Loop mediated isothermal amplification (LAMP),Is it a cost-effective alternative for PCR?

Loop mediated isothermal amplification (LAMP) is a novel technique first developed in the year 2000 by Notomi et al and further improved by Nagamine et al.1,2 The simplicity of the procedure belies the complex chemical and molecular processes that are involved in the reaction. A detailed explanation of the mechanism is beyond the scope of this article, but very briefly, it involves six primers targeting eight different areas of the target sequence, which are amplified by a DNA polymerase under isothermal (usually 60-65oC) conditions.

The Council – 2020/2021

President Prof. Nadira Karunaweera   President Elect Dr. Geethika Patabendige   Vice President Dr. Rohini Wadanamby   Immediate Past President Dr. N. Shirani Chandrasiri   Hon. Secretaries              Dr. Madhumanee Abeywardena Dr. Nilakshi Samaranayake   Hon. Treasurer Dr. Nadisha Badanasinghe   Editor Prof. Neluka Fernando  …

Laboratory diagnosis of toxoplasmosis: methods, pitfalls and new developments

By Nilakshi Samaranayake MBBS, Dip Med Micro, PhD Senior Lecturer in Parasitology, Faculty of Medicine, University of Colombo Introduction Toxoplasmosis is caused by an obligate intracellular protozoan parasite, Toxoplasma gondii.  It is a typical zoonotic infection with cats and other larger felids as primary hosts…

A to Z on antimicrobial prescription

  By Dr Wadanamby J.M.R.W.W Head of department of Microbiolog Lanka hospital diagnostics 0777258366 rohiniw@lhd.lk rohiniwadanamby@gmail.com       Antimicrobial resistance is a major global threat that we encountered during this decade. Many organizations, societies, committees individually and collectively are trying to combat this threat…