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oration

The Siri Wickremesinghe Memorial Oration

The Siri Wickremesinghe Oration was instituted by the Sri Lanka College of Microbiologists in 2004, in memory of the late Dr R S B Wickremesinghe, who was one of the earliest members of the College, and its’ President in 1994.

LAMP_Overview_1119

Loop mediated isothermal amplification (LAMP),Is it a cost-effective alternative for PCR?

Loop mediated isothermal amplification (LAMP) is a novel technique first developed in the year 2000 by Notomi et al and further improved by Nagamine et al.1,2 The simplicity of the procedure belies the complex chemical and molecular processes that are involved in the reaction. A detailed explanation of the mechanism is beyond the scope of this article, but very briefly, it involves six primers targeting eight different areas of the target sequence, which are amplified by a DNA polymerase under isothermal (usually 60-65oC) conditions.