Tagged: Members posts 2021

genesequence 0

SARS CoV-2 variant surveillance based on gene sequencing

Department of Molecular Biology, Medical Research Institute, Colombo and Department of Zoology and Environmental Sciences, University of Colombo have conducted SARS CoV-2 variant analysis based on gene sequencing data generated by Oxford Nanopore gene sequencing method since July 2021.

LAMP_Overview_1119

Loop mediated isothermal amplification (LAMP),Is it a cost-effective alternative for PCR?

Loop mediated isothermal amplification (LAMP) is a novel technique first developed in the year 2000 by Notomi et al and further improved by Nagamine et al.1,2 The simplicity of the procedure belies the complex chemical and molecular processes that are involved in the reaction. A detailed explanation of the mechanism is beyond the scope of this article, but very briefly, it involves six primers targeting eight different areas of the target sequence, which are amplified by a DNA polymerase under isothermal (usually 60-65oC) conditions.